LOCUS       pDONR™/Zeo
DISCLAIMER  Certain terms are trademarks or registered trademarks of Invitrogen 
            Corporation. See "Intellectual Property" in the Help file for more 
            information.
FEATURES             Location/Qualifiers
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence (c)"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence (c)"
     primer_bind     537..552
                     /note="M13 Forward (-20) priming site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1"
     misc_recomb     complement(1577..1673)
                     /label=attL2
                     /note="attL2 (c)"
     misc_signal     complement(1688..1707)
                     /note="T7 Promoter/priming site (c)"
     primer_bind     1715..1731
                     /note="M13 Reverse priming site"
     gene            1844..2653
                     /note="Kanamycin resistance gene"
     rep_origin      2774..3447
                     /note="pUC origin"
     vector          join(1585..3450,1..651)
                     /source="pDONR%99221"
                     /type="Donor Vector"
     misc_feature    669..691
                     /note="TEV site"
     source          692..1570
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="MGC:5017 IMAGE:3449625"
                     /tissue_type="Placenta, choriocarcinoma"
                     /clone_lib="NIH_MGC_10"
                     /lab_host="DH10B"
                     /note="Vector: pCMV-SPORT6"
     gene            692..1570
                     /gene="ST3GAL4"
                     /gene_synonym=CGS23
                     /gene_synonym=FLJ11867
                     /gene_synonym=NANTA3
                     /gene_synonym=SAT3
                     /gene_synonym=SIAT4
                     /gene_synonym=SIAT4C
                     /gene_synonym=ST3Gal IV
                     /gene_synonym=ST3GalIV
                     /gene_synonym=STZ
                     /db_xref="GeneID:6484"
                     /db_xref="MIM:104240"
     CDS             692..1570
                     /dnas_title="ST3GAL4 protein"
                     /gene="ST3GAL4"
                     /gene_synonym=CGS23
                     /gene_synonym=FLJ11867
                     /gene_synonym=NANTA3
                     /gene_synonym=SAT3
                     /gene_synonym=SIAT4
                     /gene_synonym=SIAT4C
                     /gene_synonym=ST3Gal IV
                     /gene_synonym=ST3GalIV
                     /gene_synonym=STZ
                     /codon_start=1
                     /product="ST3GAL4 protein"
                     /protein_id="AAH10645.1"
                     /db_xref="GI:14714972"
                     /db_xref="GeneID:6484"
                     /db_xref="MIM:104240"
                     /translation="MVSKSRWKLLAMLALVLVVMVWYSISREDRYIELFYFPIPEKKE
                     PCLQGEAESKASKLFGNYSRDQPIFLRLEDYFWVKTPSAYELPYGTKGSEDLLLRVLA
                     ITSSSIPKNIQSLRCRRCVVVGNGHRLRNSSLGDAINKYDVVIRLNNAPVAGYEGDVG
                     SKTTMRLFYPESAHFDPKVENNPDTLLVLVAFKAMDFHWIETILSDKKRVRKGFWKQP
                     PLIWDVNPKQIRILNPFFMEIAADKLLSLPMQQPRKIKQKPTTGLLAITLALHLCDLV
                     HIAGFGYPDAYNKKQTIHYYEQITLKSMAGSGHNVSQEALAIKRMLEMGAIKNLTSF"
ORIGIN      
          1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
         61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
        121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
        181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
        241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
        301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
        361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
        421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
        481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
        541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
        601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
        661 AGCAGGCTct gaaaacttgt actttcaagg cgagaagaag gagccgtgcc tccagggtga
        721 ggcagagagc aaggcctcta agctctttgg caactactcc cgggatcagc ccatcttcct
        781 gcggcttgag gattatttct gggtcaagac gccatctgct tacgagctgc cctatgggac
        841 caaggggagt gaggatctgc tcctccgggt gctagccatc accagctcct ccatccccaa
        901 gaacatccag agcctcaggt gccgccgctg tgtggtcgtg gggaacgggc accggctgcg
        961 gaacagctca ctgggagatg ccatcaacaa gtacgatgtg gtcatcagat tgaacaatgc
       1021 cccagtggct ggctatgagg gtgacgtggg ctccaagacc accatgcgtc tcttctaccc
       1081 tgaatctgcc cacttcgacc ccaaagtaga aaacaaccca gacacactcc tcgtcctggt
       1141 agctttcaag gcaatggact tccactggat tgagaccatc ctgagtgata agaagcgggt
       1201 gcgaaagggt ttctggaaac agcctcccct catctgggat gtcaatccta aacagattcg
       1261 gattctcaac cccttcttca tggagattgc agctgacaaa ctgctgagcc tgccaatgca
       1321 acagccacgg aagattaagc agaagcccac cacgggcctg ttggccatca cgctggccct
       1381 ccacctctgt gacttggtgc acattgccgg ctttggctac ccagacgcct acaacaagaa
       1441 gcagaccatt cactactatg agcagatcac gctcaagtcc atggcggggt caggccataa
       1501 tgtctcccaa gaggccctgg ccattaagcg gatgctggag atgggagcta tcaagaacct
       1561 cacgtccttc TAGGACCCAG CTTTCTTGTA CAAAGTTGGC ATTATAAGAA AGCATTGCTT
       1621 ATCAATTTGT TGCAACGAAC AGGTCACTAT CAGTCAAAAT AAAATCATTA TTTGCCATCC
       1681 AGCTGATATC CCCTATAGTG AGTCGTATTA CATGGTCATA GCTGTTTCCT GGCAGCTCTG
       1741 GCCCGTGTCT CAAAATCTCT GATGTTACAT TGCACAAGAT AAAATAATAT CATCATGAAC
       1801 AATAAAACTG TCTGCTTACA TAAACAGTAA TACAAGGGGT GTTATGAGCC ATATTCAACG
       1861 GGAAACGTCG AGGCCGCGAT TAAATTCCAA CATGGATGCT GATTTATATG GGTATAAATG
       1921 GGCTCGCGAT AATGTCGGGC AATCAGGTGC GACAATCTAT CGCTTGTATG GGAAGCCCGA
       1981 TGCGCCAGAG TTGTTTCTGA AACATGGCAA AGGTAGCGTT GCCAATGATG TTACAGATGA
       2041 GATGGTCAGA CTAAACTGGC TGACGGAATT TATGCCTCTT CCGACCATCA AGCATTTTAT
       2101 CCGTACTCCT GATGATGCAT GGTTACTCAC CACTGCGATC CCCGGAAAAA CAGCATTCCA
       2161 GGTATTAGAA GAATATCCTG ATTCAGGTGA AAATATTGTT GATGCGCTGG CAGTGTTCCT
       2221 GCGCCGGTTG CATTCGATTC CTGTTTGTAA TTGTCCTTTT AACAGCGATC GCGTATTTCG
       2281 TCTCGCTCAG GCGCAATCAC GAATGAATAA CGGTTTGGTT GATGCGAGTG ATTTTGATGA
       2341 CGAGCGTAAT GGCTGGCCTG TTGAACAAGT CTGGAAAGAA ATGCATAAAC TTTTGCCATT
       2401 CTCACCGGAT TCAGTCGTCA CTCATGGTGA TTTCTCACTT GATAACCTTA TTTTTGACGA
       2461 GGGGAAATTA ATAGGTTGTA TTGATGTTGG ACGAGTCGGA ATCGCAGACC GATACCAGGA
       2521 TCTTGCCATC CTATGGAACT GCCTCGGTGA GTTTTCTCCT TCATTACAGA AACGGCTTTT
       2581 TCAAAAATAT GGTATTGATA ATCCTGATAT GAATAAATTG CAGTTTCATT TGATGCTCGA
       2641 TGAGTTTTTC TAATCAGAAT TGGTTAATTG GTTGTAACAC TGGCAGAGCA TTACGCTGAC
       2701 TTGACGGGAC GGCGCAAGCT CATGACCAAA ATCCCTTAAC GTGAGTTACG CGTCGTTCCA
       2761 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG
       2821 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA
       2881 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA
       2941 TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC
       3001 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG
       3061 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC
       3121 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT
       3181 ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC
       3241 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG
       3301 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG
       3361 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT
       3421 GGCCTTTTGC TGGCCTTTTG CTCACATGTT
//